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We consider the in vivo assay, in which ceramic cubes are loaded with isolated and culture-expanded putative stem cells and then implanted subcutaneously into immunocompromised host animals, to be the de nitive test of the osteogenic and chondrogenic potential of stem cells in general. However, as indicated in Section 1, cartilage formation is almost never observed when human MSCs are used in this assay. Although bone formation has been observed in diffusion chambers loaded with cultured nonhuman marrow-derived stromal cells, the frequency of bonepositive results and the rate of bone formation are higher when the same cells are implanted in ceramic vehicles [Dennis and Caplan, 1993]. Our laboratory routinely uses biphasic ceramics consisting of 60% tricalcium phosphate and 40% hydroxyapatite, but we have also had success with coral-based ceramics. Pretreatment of the ceramics with bronectin or laminin facilitates attachment of the cells to the ceramic surface and, in turn, results in the development of bone at earlier time points than for untreated cubes [Dennis and Caplan, 1993]. Subcutaneously implanted cell-loaded ceramic cubes provide a critical control for cells used in experimental tissue engineering designs. For example, Kadiyala

Suppression of boron out-diffusion in silicon germanium HBT by carbon incorporation International Electron Device Meeting (IEDM) Technical Digest, 1996 p 249 52 46 Cowern NEB, Zalm PC, Sluis PVD, Graresteijn DJ, Groer WBD Diffusion in strained SiGe Physical Review Letters 1994;72:2585 8 47 Poate JM, Eaglesham DJ, Gilmer GH, Gossmann HJ, Jaraiz M, Rafferty CS, et al Ion implantation and transient enhanced diffusion International Electron Device Meeting (IEDM) Technical Digest, 1995 p 77 80 48 Lever RF, Bonar JM, Willoughby AFW Boron diffusion across silicon-silicon germanium boundaries Journal of Applied Physics 1999;83:1998 4 49 Rajendran K, Schoenmaker W Studies of boron diffusivity in strained Si1 xGex epitaxial layers Journal of Applied Physics 2001;89(2):980 7..

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emotion as described earlier, recent evidence points to a more fundamental association such that left frontal activation mediates approach-oriented behavior and right frontal activation is associated with avoidance or withdrawal-oriented behavior (Davidson, 2004; Jackson et al., 2003). For example, left frontal activation is manifest during hostile behavior, which is certainly not a positive affective state but most definitely involves approach toward an intended target. Whether positive in nature, approach-oriented, or a combination of the two, it would appear that such a neurobiological state would be highly adaptive for the soldier, who must control his or her arousal during active engagement with challenging tasks while under great pressure. Because EEG alpha power is inversely related to activation (i.e., relaxation), R L alpha power (Log right frontal alpha power Log left frontal alpha power), a positive value for this metric implies greater relaxation in the right region, or, in other words, a relative state of left frontal activation. Hence, positive numbers for this metric imply left activation and executive control over emotion structures and processes. Conversely, a negative value implies greater relaxation in the left region and a lack of executive control over limbic circuits. Therefore, this EEG metric provides an opportune target for neurofeedback training to enable a heightened level of executive control over emotional response and task engagement during challenge. Model of Stress-Induced Cortical Dynamics Figure 4.16 provides a schematic model of the processes and outcomes underlying stress reactivity and integrates affective and cognitive activity with psychomotor perfor-

if mainstream twentieth century social science turned its attention to African Americans, it tended to stress the ineffectualness of the adaptive abilities of ethnic minority people (Thomas & Sillen, 1972). Typical were such comments as those of the psychiatrists Kardiner and Ovesey (1951/1962). Impressed with the debilitating psychological effects that centuries of American racism had on African Americans, they concluded that the Negro has no possible basis for a healthy self-esteem (p. 297). Crain and Weisman (1972) in their large scale study of northern Black adults noted their

Beck RW (2000) A Chronology of Microbiology in Historical Context. American Society of Microbiology.

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